Isingeniso kuma-Chelates Amaminerali Amancane E-Peptide Trace
Ingxenye 1 Umlando Wezithasiselo Zamaminerali Ezilandelekayo
Ingahlukaniswa izizukulwane ezine ngokuya ngentuthuko yezithasiselo zamaminerali alula:
Isizukulwane sokuqala: Usawoti we-inorganic wamaminerali angaphansi, njenge-copper sulfate, i-ferrous sulfate, i-zinc oxide, njll; Isizukulwane sesibili: Usawoti we-organic acid wamaminerali angaphansi, njenge-ferrous lactate, i-ferrous fumarate, i-copper citrate, njll.; Isizukulwane sesithathu: I-Amino acid chelate feed grade yamaminerali angaphansi, njenge-zinc methionine, i-iron glycine kanye ne-zinc glycine; Isizukulwane sesine: Usawoti we-protein kanye nosawoti we-peptide chelating wamaminerali angaphansi, njenge-protein copper, i-protein iron, i-protein zinc, i-protein manganese, i-peptide encane ithusi, i-peptide encane insimbi, i-peptide encane i-zinc, i-peptide encane i-manganese, njll.
Isizukulwane sokuqala singamaminerali angabonakali, kanti isizukulwane sesibili kuya kwesesine singamaminerali angabonakali.
Ingxenye 2 Kungani Kufanele Ukhethe Ama-Chelate Amancane E-Peptide
Ama-chelate amancane e-peptide asebenza kahle kanje:
1. Uma ama-peptide amancane exubana nama-ion ensimbi, acebile ngezindlela futhi kunzima ukuwagcwalisa;
2. Ayincintisani neziteshi ze-amino acid, inezindawo eziningi zokumunca kanye nesivinini sokumunca okusheshayo;
3. Ukusetshenziswa kwamandla okuncane; 4. Amadiphozithi amaningi, izinga eliphezulu lokusetshenziswa kanye nokusebenza kahle kokukhiqiza izilwane okuthuthukisiwe kakhulu;
5. Ilwa namagciwane kanye ne-antioxidant;
6. Ukulawulwa kwamasosha omzimba.
Ucwaningo oluningi luye lwabonisa ukuthi izici noma imiphumela engenhla yama-chelate amancane e-peptide iwenza abe namathuba amaningi okusetshenziswa kanye namathuba okuthuthukiswa, ngakho-ke inkampani yethu ekugcineni yanquma ukuthatha ama-chelate amancane e-peptide njengokugxila kocwaningo lwenkampani ngemikhiqizo yamaminerali e-organic trace kanye nentuthuko.
Ingxenye 3 Ukusebenza kahle kwama-chelate amancane e-peptide
1. Ubudlelwano phakathi kwama-peptide, ama-amino acid kanye namaprotheni
Isisindo sama-molecule eprotheyini singaphezu kuka-10000;
Isisindo sama-molecule se-peptide singu-150 ~ 10000;
Ama-peptide amancane, abizwa nangokuthi ama-peptide amancane ama-molecule, aqukethe ama-amino acid angu-2 ~ 4;
Isisindo sama-molecule esimaphakathi sama-amino acid singaba ngu-150.
2. Ukuxhumanisa amaqembu ama-amino acid nama-peptide axutshwe nezinsimbi
(1) Amaqembu axhumanisayo kuma-amino acid
Amaqembu okuxhumanisa kuma-amino acid:
Amaqembu e-amino kanye ne-carboxyl ku-a-carbon;
Amaqembu e-side chain amanye ama-a-amino acid, njengeqembu le-sulfhydryl le-cysteine, iqembu le-phenolic le-tyrosine kanye neqembu le-imidazole le-histidine.
(2) Amaqembu axhumanisayo kuma-peptide amancane
Ama-peptide amancane anamaqembu amaningi okuxhumanisa kunama-amino acid. Uma ehlangana nama-ion ensimbi, kulula ukuwahlanganisa, futhi angakha i-chelation enama-dentate amaningi, okwenza i-chelate izinze kakhudlwana.
3. Ukusebenza kahle komkhiqizo we-peptide chelate encane
Isisekelo semfundiso ye-peptide encane ekhuthaza ukumuncwa kwamaminerali amancane
Izici zokumunca zama-peptide amancane ziyisisekelo semfundiso yokukhuthaza ukumunca kwezinto ezilandelanayo. Ngokusho kwemfundiso yendabuko ye-protein metabolism, lokho izilwane ezikudingayo ngeprotheni yilokho ezikudingayo ngama-amino acid ahlukahlukene. Kodwa-ke, eminyakeni yamuva nje, izifundo zibonise ukuthi isilinganiso sokusetshenziswa kwama-amino acid ekudleni okuvela emithonjeni ehlukene sihlukile, futhi lapho izilwane zondliwa ngokudla okune-homozygous noma ukudla okune-amino acid ephansi yeprotheni, ukusebenza okuhle kakhulu kokukhiqiza akutholakali (Baker, 1977; Pinchasov et al., 1990) [2,3]. Ngakho-ke, ezinye izazi zibeka umbono wokuthi izilwane zinamandla akhethekile okumunca iprotheni engaphelele noma ama-peptide ahlobene. U-Agar (1953)[4] waqala waphawula ukuthi umgudu wamathumbu ungamunca futhi uthuthe i-diglycidyl ngokuphelele. Kusukela ngaleso sikhathi, abacwaningi babeke impikiswano ekholisayo yokuthi ama-peptide amancane angamuncwa ngokuphelele, okuqinisekisa ukuthi i-glycylglycine engaphelele iyathuthwa futhi imuncwe; Inani elikhulu lama-peptide amancane lingamuncwa ngqo ekujikelezeni kwegazi ngendlela yama-peptide. UHara et al. (1984)[5] uphinde waveza ukuthi imikhiqizo yokugcina yokugaya ukudla yamaprotheni endleleni yokugaya ukudla ikakhulukazi ama-peptide amancane kune-amino acid yamahhala (FAA). Ama-peptide amancane angadlula ngokuphelele kumaseli e-mucosal amathumbu futhi angene ekujikelezeni kwegazi okujwayelekile (Le Guowei, 1996)[6].
Inqubekela phambili Yocwaningo Lwe-Peptide Encane Ekhuthaza Ukumuncwa Kwamaminerali Ambalwa, uQiao Wei, et al.
Ama-chelate amancane e-peptide athuthwa futhi amuncwa ngesimo sama-peptide amancane
Ngokwendlela yokumunca kanye nokuthutha kanye nezici zama-peptide amancane, amaminerali amancane ahlangana nama-peptide amancane njengoba ama-ligand amakhulu angathuthwa wonke, okuyinto ekhuthaza kakhulu ukuthuthukiswa kwamandla ebhayoloji amaminerali amancane. (Qiao Wei, et al)
Ukusebenza kahle kwe-Small Peptide Chelates
1. Uma ama-peptide amancane exubana nama-ion ensimbi, acebile ngezindlela futhi kunzima ukuwagcwalisa;
2. Ayincintisani neziteshi ze-amino acid, inezindawo eziningi zokumunca kanye nesivinini sokumunca okusheshayo;
3. Ukusetshenziswa kwamandla okuncane;
4. Amadiphozithi engeziwe, izinga eliphezulu lokusetshenziswa kanye nokusebenza kahle kokukhiqiza izilwane okuthuthukisiwe kakhulu;
5. Ilwa namagciwane kanye ne-antioxidant; 6. Ukulawulwa kwamasosha omzimba.
4. Ukuqonda okwengeziwe ngama-peptide
Yimuphi kubasebenzisi ababili be-peptide othola inzuzo enkulu?
- I-peptide ebophayo
- I-Phosphopeptide
- Ama-reagent ahlobene
- I-peptide yokulwa namagciwane
- I-peptide yomzimba
- I-Neuropeptide
- I-peptide yama-hormone
- I-peptide ye-antioxidant
- Ama-peptide okudla okunempilo
- Ama-peptides okufaka izinongo
(1) Ukuhlukaniswa kwama-peptide
(2) Imiphumela yomzimba yama-peptide
- 1. Lungisa ibhalansi yamanzi kanye ne-electrolyte emzimbeni;
- 2. Yenza ama-antibodies alwa namagciwane kanye nokutheleleka ukuze isimiso somzimba sokuzivikela sithuthukise ukusebenza kwamasosha omzimba;
- 3. Khuthaza ukuphulukiswa kwenxeba; Ukulungiswa okusheshayo kokulimala kwezicubu ze-epithelial.
- 4. Ukwenza ama-enzyme emzimbeni kusiza ukuguqula ukudla kube amandla;
- 5. Ukulungisa amaseli, ukuthuthukisa ukusebenza kahle kwamaseli, ukuvimbela ukuwohloka kwamaseli, futhi kudlala indima ekuvimbeleni umdlavuza;
- 6. Ukukhuthaza ukwenziwa nokulawulwa kwamaprotheni nama-enzyme;
- 7. Isithunywa esibalulekile samakhemikhali sokudlulisa ulwazi phakathi kwamaseli nezitho zomzimba;
- 8. Ukuvimbela izifo zenhliziyo nemithambo yegazi;
- 9. Lawula izinhlelo ze-endocrine kanye nezezinzwa.
- 10. Thuthukisa uhlelo lokugaya ukudla futhi welaphe izifo zesisu ezingamahlalakhona;
- 11. Ukuthuthukisa isifo sikashukela, isifo samathambo, isifo samathambo nezinye izifo.
- 12. Ukutheleleka okulwa namagciwane, ukulwa nokuguga, ukususwa kwama-free radical amaningi emzimbeni.
- 13. Ukukhuthaza ukusebenza kwe-hematopoietic, ukwelapha i-anemia, ukuvimbela ukuhlangana kwama-platelet, okungathuthukisa amandla okuthwala umoya-mpilo wamaseli abomvu egazi.
- 14. Ukulwa ngqo namagciwane e-DNA kanye namagciwane ahlasela amagciwane.
5. Umsebenzi wokudla okubili wama-chelates amancane e-peptide
I-chelate encane ye-peptide ingena esitokisini iyonke emzimbeni wesilwane, besebese iphula ngokuzenzakalelayo isibopho se-chelationeseli bese ibola ibe ama-ion e-peptide kanye ne-metal, asetshenziswa ngokulandelana yi-isilwane sokudlala imisebenzi emibili yokudla okunempilo, ikakhulukazi i-indima yokusebenza kwe-peptide.
Umsebenzi we-peptide encane
- 1. Khuthaza ukwakheka kwamaprotheni ezicutshini zemisipha yezilwane, unciphise i-apoptosis, futhi ukhuthaze ukukhula kwezilwane
- 2. Thuthukisa isakhiwo sezitshalo zamathumbu futhi ukhuthaze impilo yamathumbu
- 3. Nikeza uhlaka lwekhabhoni futhi wandise umsebenzi wama-enzyme okugaya ukudla njenge-amylase yamathumbu kanye ne-protease
- 4.Inemiphumela yokucindezeleka yokulwa ne-oxidative
- 5. Unezakhiwo zokulwa nokuvuvukala
- 6.……
6. Izinzuzo zama-chelate amancane e-peptide kune-chelate yama-amino acid
| Amaminerali amancane e-amino acid chelated | Amaminerali amancane e-peptide chelated trace | |
| Izindleko zezinto ezingavuthiwe | Izinto zokusetshenziswa ze-amino acid eyodwa ziyabiza kakhulu | Izinto zokusetshenziswa ze-keratin zaseShayina ziningi. Izinwele, izinselo nezimpondo ekufuyweni kwezilwane kanye namanzi angcolile amaprotheni kanye nezinsalela zesikhumba embonini yamakhemikhali ziyizinto zokusetshenziswa zeprotheni ezisezingeni eliphezulu nezishibhile. |
| Umphumela wokumunca | Amaqembu e-amino kanye ne-carboxyl ahilelekile ngesikhathi esisodwa ekuhlanganiseni kwama-amino acid kanye nezakhi zensimbi, akha isakhiwo se-bicyclic endocannabinoid esifana nesama-dipeptide, ngaphandle kwamaqembu e-carboxyl akhululekile akhona, angamuncwa kuphela ngohlelo lwe-oligopeptide. (Su Chunyang et al., 2002) | Uma ama-peptide amancane ehlanganyela ku-chelation, isakhiwo se-chelation esiyingini esisodwa ngokuvamile sakhiwa yiqembu lama-amino eliyi-terminal kanye ne-peptide bond oxygen eseduze, kanti i-chelate igcina iqembu le-carboxyl yamahhala, elingamuncwa ngohlelo lwe-dipeptide, ngamandla okumuncwa aphezulu kakhulu kunohlelo lwe-oligopeptide. |
| Ukuzinza | Ama-ion ensimbi anendandatho eyodwa noma ngaphezulu enamalungu amahlanu noma ayisithupha yamaqembu ama-amino, amaqembu e-carboxyl, amaqembu e-imidazole, amaqembu e-phenol, kanye namaqembu e-sulfhydryl. | Ngaphezu kwamaqembu amahlanu akhona okuhlanganisa ama-amino acid, amaqembu e-carbonyl nama-imino kuma-peptide amancane nawo angabandakanyeka ekuxhumaneni, okwenza ama-chelate amancane e-peptide azinze kakhulu kune-amino acid chelates. (Yang Pin et al., 2002) |
7. Izinzuzo zama-chelate amancane e-peptide kune-glycolic acid kanye nama-chelate e-methionine
| Amaminerali aqukethe i-glycine chelated trace | Amaminerali amancane e-methionine chelated | Amaminerali amancane e-peptide chelated trace | |
| Ifomu lokuxhumanisa | Amaqembu e-carboxyl nama-amino e-glycine angahlanganiswa nama-ion ensimbi. | Amaqembu e-methionine e-carboxyl kanye nama-amino angahlanganiswa nama-ion ensimbi. | Uma ixutshwe nama-ion ensimbi, icebile ngezindlela zokuhlanganisa futhi ayigcwali kalula. |
| Umsebenzi wokudla okunempilo | Izinhlobo nemisebenzi yama-amino acid iyafana. | Izinhlobo nemisebenzi yama-amino acid iyafana. | Iizinhlobo eziningi ezicebileAma-amino acid anikeza ukudla okunomsoco okuphelele, kuyilapho ama-peptide amancane engasebenza ngokufanele. |
| Umphumela wokumunca | Ama-chelate e-Glycine anayonoAmaqembu e-carboxyl akhululekile akhona futhi anomphumela wokumunca kancane. | Ama-chelate e-methionine anayonoAmaqembu e-carboxyl akhululekile akhona futhi anomphumela wokumunca kancane. | Ama-chelate amancane e-peptide akhiwequkathaukuba khona kwamaqembu e-carboxyl amahhala futhi kunomphumela wokumunca okusheshayo. |
Ingxenye 4 Igama Lokuhweba “Ama-Chelates Amancane Amaminerali Angama-Peptide”
Ama-Chelates amancane e-Peptide-mineral, njengoba igama lisho, kulula ukuwahlanza.
Kusho ama-peptide ligand amancane, angagcwali kalula ngenxa yenani elikhulu lamaqembu axhumanisayo, Kulula ukwakha i-multidentate chelate enezinto zensimbi, enokuqina okuhle.
Ingxenye 5 Isingeniso Semikhiqizo Yochungechunge Lwe-Chelates Yama-Peptide Amancane
1. Ithusi elincane elinomugqa we-peptide trace mineral (igama lokuhweba: Ibanga Lokuphakelwa Kwe-Copper Amino Acid Chelate)
2. Insimbi encane equkethe amaminerali amancane e-peptide trace (igama lokuhweba: I-Ferrous Amino Acid Chelate Feed Grade)
3. I-zinc encane equkethe amaminerali amancane e-peptide trace (igama lokuhweba: I-Zinc Amino Acid Chelate Feed Grade)
4. I-manganese encane e-chelated mineral ye-peptide trace (igama lokuhweba: I-Manganese Amino Acid Chelate Feed Grade)
Ibanga Lokuphakelwa Kwe-Copper Amino Acid Chelate
Ibanga Lokuphakelwa Kwe-Ferrous Amino Acid Chelate
Ibanga Lokuphakela le-Zinc Amino Acid Chelate
Ibanga Lokuphakela le-Manganese Amino Acid Chelate
1. Ibanga Lokuphakelwa Kwe-Copper Amino Acid Chelate
- Igama Lomkhiqizo: Ibanga Lokuphakelwa Kwe-Copper Amino Acid Chelate
- Ukubukeka: Ama-granules aluhlaza okotshani ansundu
- Amapharamitha e-physicochemical
a) Ithusi: ≥ 10.0%
b) Ama-amino acid aphelele: ≥ 20.0%
c) Izinga le-Chelation: ≥ 95%
d) I-Arsenic: ≤ 2 mg/kg
e) Umthofu: ≤ 5 mg/kg
f) I-Cadmium: ≤ 5 mg/kg
g) Okuqukethwe komswakama: ≤ 5.0%
h) Ubuncane: Zonke izinhlayiya zidlula ku-mesh engu-20, kanye nosayizi wezinhlayiya oyinhloko ongu-60-80 mesh
n=0,1,2,... kubonisa ithusi elicwebezelayo lama-dipeptide, ama-tripeptide, nama-tetrapeptide
I-Diglycerin
Isakhiwo sama-chelates amancane e-peptide
Izici ze-Copper Amino Acid Chelate Feed Grade
- Lo mkhiqizo uyiminerali e-organic kuphela e-chelating ekhishwe yinqubo ekhethekile ye-chelating enama-peptide amancane e-enzyme yezitshalo ahlanzekile njengezinto ezi-chelating kanye nezinto ezi-trace element
- Lo mkhiqizo uzinzile ngamakhemikhali futhi unganciphisa kakhulu umonakalo wawo kumavithamini namafutha, njll.
- Ukusetshenziswa kwalo mkhiqizo kuyasiza ekuthuthukiseni ikhwalithi yokudla. Umkhiqizo umuncwa ngezindlela ezincane ze-peptide kanye ne-amino acid, kunciphisa ukuncintisana kanye nokuphikisana nezinye izakhi zokulandelela, futhi unezinga elihle kakhulu lokumuncwa kanye nokusetshenziswa kwe-bio.
- Ithusi liyingxenye eyinhloko yamaseli abomvu egazi, izicubu ezixhumeneyo, amathambo, ahilelekile emzimbeni wezinhlobo ezahlukene zama-enzyme, athuthukisa umsebenzi wokuzivikela womzimba, umphumela wama-antibiotic, angandisa ukukhuluphala kwansuku zonke, athuthukise umvuzo wokudla.
Ukusetshenziswa kanye nokusebenza kahle kwe-Copper Amino Acid Chelate Feed Grade
| Into yohlelo lokusebenza | Isilinganiso esiphakanyisiwe (izinto ezigcwele i-g/t) | Okuqukethwe okuphakelayo okunenani eligcwele (mg/kg) | Ukusebenza kahle |
| Hlwanyela | 400～700 | 60～105 | 1. Thuthukisa ukusebenza kokuzala kanye neminyaka yokusebenzisa yengulube eyizinsikazi; 2. Khulisa amandla ezinsana kanye nezingulube ezincane; 3. Thuthukisa amasosha omzimba kanye nokumelana nezifo. |
| Ingulube encane | 300～600 | 45～90 | 1. Iwusizo ekuthuthukiseni imisebenzi ye-hematopoietic kanye neyokuzivikela komzimba, ithuthukise ukumelana nokucindezeleka kanye nokumelana nezifo; 2. Khulisa izinga lokukhula futhi uthuthukise kakhulu ukusebenza kahle kokudla. |
| Izingulube ezikhuluphalisa | 125 | Januwari 18.5 | |
| Inyoni | 125 | Januwari 18.5 | 1. Thuthukisa ukumelana nokucindezeleka futhi unciphise ukufa; 2. Thuthukisa isinxephezelo sokudla futhi wandise izinga lokukhula. |
| Izilwane zasemanzini | Inhlanzi 40～70 | 6～10.5 | 1. Khuthaza ukukhula, thuthukisa isinxephezelo sokudla; 2. Ukulwa nokucindezeleka, ukunciphisa ukugula nokufa. |
| I-Shrimp 150～200 | 22.5～30 | ||
| Isilwane esidla inyama ngosuku olulodwa | Januwari 0.75 | 1. Vimbela ukuguquguquka kwamalunga e-tibial, ukuphazamiseka kokunyakaza "komhlane ogobile", ukuxega, ukulimala kwemisipha yenhliziyo; 2. Vimbela ukwakheka kwezinwele noma uboya, zibe yizinwele eziqinile, zilahlekelwe ukugoba okuvamile, zivimbele ukuvela “kwamabala ampunga” esiyingini samehlo; 3. Vimbela ukwehla kwesisindo, uhudo, ukukhiqizwa kobisi Ukuncipha. |
2. Ibanga Lokuphakelwa Kwe-Ferrous Amino Acid Chelate
- Igama Lomkhiqizo: Ibanga Lokuphakelwa Kwe-Ferrous Amino Acid Chelate
- Ukubukeka: Ama-granules aluhlaza okotshani ansundu
- Amapharamitha e-physicochemical
a) Insimbi: ≥ 10.0%
b) Ama-amino acid aphelele: ≥ 19.0%
c) Izinga le-Chelation: ≥ 95%
d) I-Arsenic: ≤ 2 mg/kg
e) Umthofu: ≤ 5 mg/kg
f) I-Cadmium: ≤ 5 mg/kg
g) Okuqukethwe komswakama: ≤ 5.0%
h) Ubuncane: Zonke izinhlayiya zidlula ku-mesh engu-20, kanye nosayizi wezinhlayiya oyinhloko ongu-60-80 mesh
n=0,1,2,...ibonisa i-zinc e-chelated yama-dipeptide, ama-tripeptide, nama-tetrapeptide
Izici ze-Ferrous Amino Acid Chelate Feed Grade
- Lo mkhiqizo uyiminerali e-organic trace chelated yizinqubo ezikhethekile ze-chelating ezinama-peptide amancane e-enzyme yezitshalo ahlanzekile njengezinto ezi-chelating kanye nezinto ezi-trace;
- Lo mkhiqizo uzinzile ngamakhemikhali futhi unganciphisa kakhulu umonakalo wawo kumavithamini namafutha, njll. Ukusetshenziswa kwalo mkhiqizo kuyasiza ekuthuthukiseni ikhwalithi yokudla;
- Umkhiqizo umuncwa ngezindlela ezincane ze-peptide kanye ne-amino acid, kunciphisa ukuncintisana kanye nokuphikisana nezinye izakhi zokulandelela, futhi unezinga elihle kakhulu lokumuncwa kanye nokusetshenziswa kwe-bio;
- Lo mkhiqizo ungadlula esivimbelweni se-placenta kanye ne-mammary gland, wenze umbungu ube nempilo enhle, wandise isisindo sokuzalwa kanye nesisindo sokulunyulwa, futhi wehlise izinga lokufa; I-iron iyisici esibalulekile se-hemoglobin kanye ne-myoglobin, okungavimbela ngempumelelo i-anemia yokuntuleka kwe-iron kanye nezinkinga zayo.
Ukusetshenziswa kanye nokusebenza kahle kwe-Ferrous Amino Acid Chelate Feed Grade
| Into yohlelo lokusebenza | Isilinganiso esiphakanyisiwe (izinto eziphelele ze-g/t) | Okuqukethwe okuphakelayo okunenani eligcwele (mg/kg) | Ukusebenza kahle |
| Hlwanyela | 300～800 | 45～120 | 1. Thuthukisa ukusebenza kokuzala kanye nokuphila kokusetshenziswa kwezingulube ezinsikazi; 2. ukuthuthukisa leso sisindo sokuzalwa, isisindo sokulunyulwa kanye nokufana kwengulube encane ukuze kukhiqizwe kangcono esikhathini esizayo; 3. Thuthukisa ukugcinwa kwensimbi ezingulubeni ezincelisayo kanye nokugcwala kwensimbi ebisini ukuze kuvinjelwe i-anemia yokuntuleka kwensimbi ezingulubeni ezincelisayo. |
| Amachwane nezingulube ezikhuluphalisa | Amachwane engulube angu-300～600 | 45～90 | 1. Ukuthuthukisa amasosha omzimba ezingulube ezincane, ukuthuthukisa ukumelana nezifo kanye nokuthuthukisa izinga lokusinda; 2. Khulisa izinga lokukhula, thuthukisa ukuguqulwa kokudla, khulisa isisindo sokulahla udoti kanye nokufana kwawo, futhi unciphise ukwanda kwezifo zezingulube; 3. Thuthukisa izinga le-myoglobin kanye ne-myoglobin, uvimbele futhi welaphe i-anemia yokuntuleka kwe-iron, wenze isikhumba sengulube sibe bomvu futhi kusobala ukuthi sithuthukise umbala wenyama. |
| Izingulube ezikhuluphalisa 200～400 | 30～60 | ||
| Inyoni | 300～400 | 45～60 | 1. Thuthukisa ukuguqulwa kokudla, ukwandisa izinga lokukhula, ukuthuthukisa ikhono lokulwa nokucindezeleka nokunciphisa ukufa; 2. Thuthukisa izinga lokubeka amaqanda, wehlise izinga lokwaphuka kwamaqanda bese ujulisa umbala we-yolk; 3. Thuthukisa izinga lokuzala kanye nezinga lokuchamisela amaqanda okuzala kanye nezinga lokusinda kwezinkukhu ezisencane. |
| Izilwane zasemanzini | 200～300 | 30～45 | 1. Khuthaza ukukhula, thuthukisa ukuguqulwa kokudla; 2. Thuthukisa amandla okulwa nokucindezeleka, nciphisa ukugula nokufa. |
3. Ibanga Lokuphakelwa le-Zinc Amino Acid Chelate
- Igama Lomkhiqizo: Ibanga Lokuphakelayo le-Zinc Amino Acid Chelate
- Ukubukeka: ama-granules ansundu-aphuzi
- Amapharamitha e-physicochemical
a) I-Zinc: ≥ 10.0%
b) Ama-amino acid aphelele: ≥ 20.5%
c) Izinga le-Chelation: ≥ 95%
d) I-Arsenic: ≤ 2 mg/kg
e) Umthofu: ≤ 5 mg/kg
f) I-Cadmium: ≤ 5 mg/kg
g) Okuqukethwe komswakama: ≤ 5.0%
h) Ubuncane: Zonke izinhlayiya zidlula ku-mesh engu-20, kanye nosayizi wezinhlayiya oyinhloko ongu-60-80 mesh
n=0,1,2,...ibonisa i-zinc e-chelated yama-dipeptide, ama-tripeptide, nama-tetrapeptide
Izici ze-Zinc Amino Acid Chelate Feed Grade
Lo mkhiqizo uyiminerali e-organic kuphela e-chelated yizinqubo ezikhethekile ze-chelating ezinama-peptide amancane e-enzyme yezitshalo ahlanzekile njengezinto ezi-chelating kanye nezinto ezi-trace;
Lo mkhiqizo uzinzile ngamakhemikhali futhi unganciphisa kakhulu umonakalo wawo kumavithamini namafutha, njll.
Ukusetshenziswa kwalo mkhiqizo kuyasiza ekuthuthukiseni ikhwalithi yokudla; Umkhiqizo umuncwa ngezindlela ezincane ze-peptide kanye ne-amino acid, kunciphisa ukuncintisana kanye nokuphikisana nezinye izakhi zokulandelela, futhi unezinga elihle kakhulu lokumuncwa kanye nokusetshenziswa kwe-bio;
Lo mkhiqizo ungathuthukisa amasosha omzimba, ukhuthaze ukukhula, wandise ukuguqulwa kokudla futhi uthuthukise ukucwebezela koboya;
I-Zinc iyisakhi esibalulekile sama-enzyme angaphezu kuka-200, izicubu ze-epithelial, i-ribose kanye ne-gustatin. Ikhuthaza ukwanda okusheshayo kwamaseli e-tastebud ku-mucosa yolimi futhi ilawula isifiso sokudla; ivimbela amabhaktheriya ayingozi emathunjini; futhi inomsebenzi wama-antibiotic, angathuthukisa umsebenzi wokukhipha uketshezi lwesimiso sokugaya ukudla kanye nomsebenzi wama-enzyme ezicutshini nasemaseli.
Ukusetshenziswa kanye nokusebenza kahle kwe-Zinc Amino Acid Chelate Feed Grade
| Into yohlelo lokusebenza | Isilinganiso esiphakanyisiwe (izinto eziphelele ze-g/t) | Okuqukethwe okuphakelayo okunenani eligcwele (mg/kg) | Ukusebenza kahle |
| Izinyoni ezikhulelwe nezincelisayo | 300～500 | 45～75 | 1. Thuthukisa ukusebenza kokuzala kanye nokuphila kokusetshenziswa kwezingulube ezinsikazi; 2. Thuthukisa amandla engane encane kanye namachwane, uthuthukise ukumelana nezifo, futhi ubenze babe nokusebenza kahle kokukhiqiza esigabeni esilandelayo; 3. Thuthukisa isimo somzimba sezingulube ezikhulelwe kanye nesisindo sokuzalwa kwezingulube ezincane. |
| Ingulube encane encelayo, ingulube encane kanye nezingulube ezikhulisayo | 250～400 | 37.5～60 | 1. Ukuthuthukisa amasosha omzimba ezingulube ezincane, ukunciphisa isifo sohudo kanye nokufa; 2. Ukuthuthukisa ukunambitheka, ukwandisa ukudla okudliwayo, ukwandisa izinga lokukhula kanye nokuthuthukisa ukuguqulwa kokudla; 3. Yenza uboya bengulube bukhanye futhi uthuthukise ikhwalithi yesidumbu kanye nekhwalithi yenyama. |
| Inyoni | 300～400 | 45～60 | 1. Thuthukisa ukukhazimula kwezimpaphe; 2. ukuthuthukisa izinga lokubeka amaqanda, izinga lokukhulelwa kanye nezinga lokuchamisela amaqanda, kanye nokuqinisa ikhono lokufaka imibala leqanda eliphuzi; 3. Thuthukisa ikhono lokulwa nokucindezeleka futhi unciphise ukufa; 4. Thuthukisa ukuguqulwa kokudla futhi wandise izinga lokukhula. |
| Izilwane zasemanzini | Januwari 300 | 45 | 1. Khuthaza ukukhula, thuthukisa ukuguqulwa kokudla; 2. Thuthukisa amandla okulwa nokucindezeleka, nciphisa ukugula nokufa. |
| Isilwane esidla inyama ngosuku olulodwa | 2.4 | 1. Thuthukisa ukukhiqizwa kobisi, uvimbele i-mastitis kanye nokubola kwe-foof, futhi unciphise okuqukethwe kwamaseli omzimba obisini; 2. Khuthaza ukukhula, thuthukisa ukuguqulwa kokudla kanye nokuthuthukisa ikhwalithi yenyama. |
4. Ibanga Lokuphakelwa Kwe-Manganese Amino Acid Chelate
- Igama Lomkhiqizo: Ibanga Lokuphakelayo le-Manganese Amino Acid Chelate
- Ukubukeka: ama-granules ansundu-aphuzi
- Amapharamitha e-physicochemical
a) Mn: ≥ 10.0%
b) Ama-amino acid aphelele: ≥ 19.5%
c) Izinga le-Chelation: ≥ 95%
d) I-Arsenic: ≤ 2 mg/kg
e) Umthofu: ≤ 5 mg/kg
f) I-Cadmium: ≤ 5 mg/kg
g) Okuqukethwe komswakama: ≤ 5.0%
h) Ubuncane: Zonke izinhlayiya zidlula ku-mesh engu-20, kanye nosayizi wezinhlayiya oyinhloko ongu-60-80 mesh
n=0, 1,2,...ibonisa i-manganese e-chelated yama-dipeptides, ama-tripeptides, nama-tetrapeptides
Izici ze-Manganese Amino Acid Chelate Feed Grade
Lo mkhiqizo uyiminerali e-organic kuphela e-chelated yizinqubo ezikhethekile ze-chelating ezinama-peptide amancane e-enzyme yezitshalo ahlanzekile njengezinto ezi-chelating kanye nezinto ezi-trace;
Lo mkhiqizo uzinzile ngamakhemikhali futhi unganciphisa kakhulu umonakalo wawo kumavithamini namafutha, njll. Ukusetshenziswa kwalo mkhiqizo kuyasiza ekuthuthukiseni ikhwalithi yokudla;
Umkhiqizo umuncwa ngezindlela ezincane ze-peptide kanye ne-amino acid, kunciphisa ukuncintisana kanye nokuphikisana nezinye izakhi zokulandelela, futhi unezinga elihle kakhulu lokumuncwa kanye nokusetshenziswa kwe-bio;
Umkhiqizo ungathuthukisa izinga lokukhula, uthuthukise ukuguqulwa kokudla kanye nesimo sempilo kakhulu; futhi uthuthukise izinga lokubeka amaqanda, izinga lokuchamisela kanye nezinga elinempilo lokuzalisa izinkukhu ngokusobala;
I-Manganese iyadingeka ekukhuleni kwamathambo kanye nokugcinwa kwezicubu ezixhumene. Ihlobene eduze nama-enzyme amaningi; futhi ihlanganyela ekugayweni kwama-carbohydrate, amafutha kanye namaprotheni, ukuzala kanye nokusabela komzimba.
Ukusetshenziswa kanye nokusebenza kahle kwe-Manganese Amino Acid Chelate Feed Grade
| Into yohlelo lokusebenza | Isilinganiso esiphakanyisiwe (izinto ezigcwele i-g/t) | Okuqukethwe okuphakelayo okunenani eligcwele (mg/kg) | Ukusebenza kahle |
| Ingulube yokuzalanisa | 200～300 | 30～45 | 1. Ukukhuthaza ukukhula okuvamile kwezitho zobulili nokuthuthukisa ukuhamba kwesidoda; 2. Thuthukisa amandla okuzala ezingulubeni futhi unciphise izithiyo zokuzala. |
| Amachwane nezingulube ezikhuluphalisa | 100～250 | 15～37.5 | 1. Kuwusizo ukuthuthukisa imisebenzi yokuzivikela komzimba, nokuthuthukisa ikhono lokulwa nokucindezeleka kanye nokumelana nezifo; 2. Khuthaza ukukhula nokuthuthukisa ukuguqulwa kokudla kakhulu; 3. Thuthukisa umbala nekhwalithi yenyama, futhi uthuthukise iphesenti lenyama engenamafutha. |
| Inyoni | 250～350 | 37.5～52.5 | 1. Thuthukisa ikhono lokulwa nokucindezeleka futhi unciphise ukufa; 2. Thuthukisa izinga lokubeka amaqanda, izinga lokukhulelwa kanye nezinga lokuchamisela amaqanda, thuthukisa ikhwalithi yamagobolondo amaqanda futhi unciphise izinga lokuphuka kwamagobolondo; 3. Khuthaza ukukhula kwamathambo futhi unciphise ukwanda kwezifo zemilenze. |
| Izilwane zasemanzini | 100～200 | 15～30 | 1. Khuthaza ukukhula nokuthuthukisa ikhono layo lokulwa nokucindezeleka kanye nokumelana nezifo; 2. Thuthukisa ukuhamba kwesidoda kanye nezinga lokuchamisela kwamaqanda avundisiwe. |
| Isilwane esidla inyama ngosuku olulodwa | Izinkomo 1.25 | 1. Vimbela ukuphazamiseka kokwenziwa kwe-fatty acid kanye nokulimala kwezicubu zamathambo; 2. Thuthukisa amandla okuzala, uvimbele ukukhipha isisu kanye nokukhubazeka kwangemva kokubeletha kwezilwane zesifazane, unciphise ukufa kwamathole namawundlu, futhi kwandiswe isisindo sezilwane ezincane ezisanda kuzalwa. | |
| Imbuzi 0.25 |
Ingxenye 6 I-FAB yama-Chelates amancane e-Peptide-mineral
| S/N | F: Izimfanelo ezisebenzayo | A: Umehluko wokuncintisana | B: Izinzuzo ezilethwa ukungezwani kokuncintisana kubasebenzisi |
| 1 | Ukulawulwa kokukhetha kwezinto zokusetshenziswa | Khetha i-hydrolysis ye-enzyme yesitshalo emsulwa yama-peptide amancane | Ukuphepha okuphezulu kwezinto eziphilayo, ukugwema ukudla abantu |
| 2 | Ubuchwepheshe bokugaya obuqondisayo be-enzyme yezinto eziphilayo ezinamaphrotheni amabili | Ingxenye ephezulu yama-peptide amancane ama-molecule | "Izinhloso" ezengeziwe, okungelula ukuzigcwalisa, ezinomsebenzi ophezulu wezinto eziphilayo kanye nokuqina okungcono |
| 3 | Ubuchwepheshe obuthuthukisiwe bokufutha nokomisa ngengcindezi | Umkhiqizo ohlanganisiwe, onosayizi wezinhlayiya ezifanayo, ukugeleza okungcono, akulula ukuwumunca umswakama | Qinisekisa ukuthi kulula ukuyisebenzisa, ukuxuba okufanayo ekudleni okuphelele |
| Okuqukethwe kwamanzi aphansi (≤ 5%), okunciphisa kakhulu ithonya elibangelwa amavithamini namalungiselelo e-enzyme | Thuthukisa ukuzinza kwemikhiqizo yokuphakelayo | ||
| 4 | Ubuchwepheshe bokulawula ukukhiqiza obuthuthukisiwe | Inqubo evalwe ngokuphelele, izinga eliphezulu lokulawula okuzenzakalelayo | Ikhwalithi ephephile nezinzile |
| 5 | Ubuchwepheshe bokulawula ikhwalithi obuthuthukisiwe | Sungula futhi uthuthukise izindlela zesayensi nezithuthukisiwe zokuhlaziya kanye nezindlela zokulawula zokuthola izici ezithinta ikhwalithi yomkhiqizo, njengeprotheni encibilika ku-asidi, ukusatshalaliswa kwesisindo sama-molecule, ama-amino acid kanye nesilinganiso se-chelating | Qinisekisa ikhwalithi, qinisekisa ukusebenza kahle futhi uthuthukise ukusebenza kahle |
Ingxenye 7 Ukuqhathaniswa Komncintiswano
Izinga elijwayelekile le-VS
Ukuqhathaniswa kokusatshalaliswa kwe-peptide kanye nezinga le-chelation yemikhiqizo
| Imikhiqizo kaSustar | Isilinganiso sama-peptide amancane (180-500) | Imikhiqizo ye-Zinpro | Isilinganiso sama-peptide amancane (180-500) |
| I-AA-Cu | ≥74% | I-AVAILA-Cu | 78% |
| I-AA-Fe | ≥48% | UKUTHOLAKALA-FE | 59% |
| I-AA-Mn | ≥33% | I-AVAILA-Mn | 53% |
| I-AA-Zn | ≥37% | I-AVAILA-Zn | 56% |
| Imikhiqizo kaSustar | Izinga le-Chelation | Imikhiqizo ye-Zinpro | Izinga le-Chelation |
| I-AA-Cu | 94.8% | I-AVAILA-Cu | 94.8% |
| I-AA-Fe | 95.3% | UKUTHOLAKALA-FE | 93.5% |
| I-AA-Mn | 94.6% | I-AVAILA-Mn | 94.6% |
| I-AA-Zn | 97.7% | I-AVAILA-Zn | 90.6% |
Isilinganiso sama-peptide amancane e-Sustar siphansi kancane kunese-Zinpro, kanti izinga lokukhula kwemikhiqizo ye-Sustar liphakeme kancane kunelemikhiqizo ye-Zinpro.
Ukuqhathaniswa kokuqukethwe kwama-amino acid angu-17 emikhiqizweni ehlukene
| Igama lika ama-amino acid | Ithusi likaSustar I-Amino Acid Chelate Ibanga Lokuphakelayo | I-Zinpro's KUTHOLAKALA ithusi | I-Sustar's Ferrous Amino Acid C Okuphakelayo kwe-helate Ibanga | UKUTHOLAKALA KWE-ZINPRO insimbi | I-Manganese kaSustar I-Amino Acid Chelate Ibanga Lokuphakelayo | UKUTHOLAKALA KWE-ZINPRO i-manganese | I-Sustar's Zinc I-Amino Acid Ibanga Lokuphakela le-Chelate | UKUTHOLAKALA KWE-ZINPRO i-zinc |
| i-aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| i-glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| I-Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| I-Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| I-Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| I-Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| I-Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| I-Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| I-Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| I-Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| I-Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| I-Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Januwari 0.75 | 0.44 |
| I-Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| I-Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| I-Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| I-Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| I-Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Ama-amino acid aphelele (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Sekukonke, isilinganiso sama-amino acid emikhiqizweni kaSustar siphezulu kunesemikhiqizo kaZinpro.
Ingxenye 8 Imiphumela yokusetshenziswa
Imiphumela yemithombo ehlukene yamaminerali amancane ekusebenzeni kahle kokukhiqiza kanye nekhwalithi yamaqanda ezinkukhu ezibekela amaqanda esikhathini sokubeletha sekwephuzile
Inqubo Yokukhiqiza
- Ubuchwepheshe be-chelation obuqondiwe
- Ubuchwepheshe bokushefa i-emulsification
- Ubuchwepheshe bokufutha nokomisa ngengcindezi
- Ubuchwepheshe bokuqandisa nokususa umswakama
- Ubuchwepheshe obuthuthukisiwe bokulawula imvelo
Isithasiselo A: Izindlela Zokunquma Ukusatshalaliswa Kwesisindo Sama-peptide Esihlobene Nama-molecule
Ukwamukelwa kwendinganiso: GB/T 22492-2008
1 Isimiso Sokuhlola:
Kwanqunywa yi-chromatography yokuhlunga ijeli esebenza kahle kakhulu. Okusho ukuthi, kusetshenziswa i-porous filler njengesigaba esimile, ngokusekelwe kumehluko ngobukhulu besisindo sama-molecule esihlobene sezingxenye zesampula zokuhlukanisa, ezitholwe ku-peptide bond ye-ultraviolet absorption wavelength engu-220nm, kusetshenziswa isofthiwe yokucubungula idatha ezinikele yokunquma ukusatshalaliswa kwesisindo sama-molecule esihlobene yi-gel filtration chromatography (okungukuthi, isofthiwe ye-GPC), ama-chromatogram kanye nedatha yawo kwacutshungulwa, kwabalwa ukuthola usayizi wesisindo sama-molecule esihlobene se-soybean peptide kanye nobubanzi bokusatshalaliswa.
2. Ama-reagent
Amanzi okuhlola kufanele ahlangabezane nencazelo yamanzi esibili ku-GB/T6682, ukusetshenziswa kwama-reagent, ngaphandle kwemibandela ekhethekile, kuhlanzekile ngokuhlaziya.
2.1 Ama-reagent afaka i-acetonitrile (emsulwa ngokwe-chromatographic), i-trifluoroacetic acid (emsulwa ngokwe-chromatographic),
2.2 Izinto ezijwayelekile ezisetshenziswa ku-calibration curve yokusatshalaliswa kwesisindo sama-molecule okuhlobene: i-insulin, i-mycopeptides, i-glycine-glycine-tyrosine-arginine, i-glycine-glycine-glycine
3 Ithuluzi kanye nemishini
3.1 I-Chromatograph Yoketshezi Esebenza Kakhulu (i-HPLC): indawo yokusebenza ye-chromatographic noma i-integrator ene-UV detector kanye nesofthiwe yokucubungula idatha ye-GPC.
3.2 Iyunithi yokuhlunga nokukhipha igesi yesigaba esiphathwayo.
3.3 Ibhalansi ye-elekthronikhi: inani eliphelele 0.000 1g.
Izinyathelo zokusebenza ezi-4
4.1 Izimo ze-Chromatographic kanye nokuhlolwa kokuzivumelanisa nesistimu (izimo zokubhekisela)
4.1.1 Ikholomu ye-Chromatographic: I-TSKgelG2000swxl300 mm×7.8 mm (ububanzi bangaphakathi) noma amanye amakholomu ejeli ohlobo olufanayo anokusebenza okufanayo okufanelekela ukunqunywa kwamaprotheni nama-peptide.
4.1.2 Isigaba sokuhamba: I-Acetonitrile + amanzi + i-trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Ubude be-wavelength bokuthola: 220 nm.
4.1.4 Izinga lokugeleza: 0.5 mL/min.
4.1.5 Isikhathi sokuthola: imizuzu engama-30.
4.1.6 Umthamo womjovo wesampula: 20μL.
4.1.7 Izinga lokushisa lekholomu: izinga lokushisa legumbi.
4.1.8 Ukuze kwenziwe uhlelo lwe-chromatographic luhlangabezane nezidingo zokuthola, kwabekwa ukuthi ngaphansi kwezimo ze-chromatographic ezingenhla, ukusebenza kahle kwekholomu ye-gel chromatographic, okungukuthi, inani lamapuleti (N), kwakungengaphansi kuka-10000 okubalwa ngokusekelwe eziqongweni ze-tripeptide standard (Glycine-Glycine-Glycine).
4.2 Ukukhiqizwa kwama-curve ajwayelekile esisindo sama-molecule ahlobene
Izixazululo ezijwayelekile ze-peptide ezilinganiselwe ze-molecule mass peptide ezingenhla ezine-mass concentration engu-1 mg / mL zalungiswa ngokufanisa isigaba esiphathwayo, zaxutshwa ngesilinganiso esithile, zabe sezihlungwa nge-organic phase membrane enosayizi we-pore ongu-0.2 μm ~ 0.5 μm zafakwa kusampula, kwabe sekutholwa ama-chromatograms ezindinganiso. Ama-relative molecular mass calibration curves kanye nezibalo zawo zatholakala ngokuhlela i-logarithm ye-relative molecular mass ngokumelene nesikhathi sokugcina noma ngokuhlehla okuqondile.
4.3 Ukwelashwa kwesampula
Kala kahle isampula engu-10mg ebhodleleni elinomthamo ongu-10mL, engeza isigaba esincane esihambayo, ukushukunyiswa kwe-ultrasonic imizuzu eyi-10, ukuze isampula ichitheke ngokuphelele futhi ixutshwe, ixutshwe nesigaba esihambayo esikalini, bese ihlungwa nge-membrane yesigaba se-organic enosayizi wembobo ongu-0.2μm ~ 0.5μm, bese i-filtrate ihlaziywe ngokwezimo ze-chromatographic ku-A.4.1.
5. Ukubalwa kokusatshalaliswa kwesisindo sama-molecule okuhlobene
Ngemva kokuhlaziya ikhambi lesampula elilungiselelwe ku-4.3 ngaphansi kwezimo ze-chromatographic zika-4.1, isisindo sama-molecule esihlobene sesampula kanye nobubanzi baso bokusatshalaliswa kungatholakala ngokufaka idatha ye-chromatographic yesampula ku-calibration curve 4.2 ngesofthiwe yokucubungula idatha ye-GPC. Ukusatshalaliswa kwesisindo sama-molecule esihlobene sama-peptide ahlukene kungabalwa ngendlela yokujwayeza indawo ephakeme, ngokwefomula: X=A/A inani ×100
Kufomula: X - Ingxenye yesisindo se-peptide yesisindo sama-molecule esihlobene ku-peptide iyonke kusampula, %;
A - Indawo ephakeme ye-peptide yesisindo sama-molecule esihlobene;
Isamba A - isamba sezindawo eziphakeme ze-peptide ngayinye yesisindo sama-molecule esihlobene, esibalwa endaweni eyodwa yedesimali.
6 Ukuphindaphinda
Umehluko ophelele phakathi kwezinqumo ezimbili ezizimele ezitholwe ngaphansi kwezimo zokuphindaphindeka akufanele udlule u-15% wesilinganiso sezibalo sezinqumo ezimbili.
Isithasiselo B: Izindlela Zokunquma Ama-Amino Acids Amahhala
Ukwamukelwa kwendinganiso: Q/320205 KAVN05-2016
1.2 Ama-reagent nezinto zokwakha
I-acetic acid ye-glacial: ihlanzekile ngokwezibalo
I-Perchloric acid: 0.0500 mol/L
Inkomba: Inkomba ye-crystal violet engu-0.1% (i-glacial acetic acid)
2. Ukunqunywa kwama-amino acid amahhala
Amasampula omisiwe ku-80°C ihora eli-1.
Beka isampula esitsheni esomile ukuze iphole ngokwemvelo kuze kufike ekushiseni kwegumbi noma iphole kuze kufike ekushiseni okusebenzisekayo.
Kala cishe u-0.1 g wesampula (onembile kuya ku-0.001 g) ebhodleleni eliyindilinga elomile elingu-250 mL.
Qhubeka ngokushesha uye esinyathelweni esilandelayo ukuze ugweme ukuthi isampula ingamunci umswakama ozungezile
Engeza u-25 mL we-glacial acetic acid bese uxuba kahle kungabi ngaphezu kwemizuzu emi-5.
Engeza amaconsi amabili esibonakaliso se-crystal violet
I-Titrate enesisombululo se-titration esijwayelekile esingu-0.0500 mol / L (±0.001) se-perchloric acid kuze kube yilapho isisombululo sishintsha kusuka kobubende kuya ekugcineni.
Qopha umthamo wesisombululo esijwayelekile esisetshenzisiwe.
Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
3. Ukubala kanye nemiphumela
Okuqukethwe kwe-amino acid yamahhala u-X ku-reagent kuvezwa njengengxenye enkulu (%) futhi kubalwa ngokwefomula: X = C × (V1-V0) × 0.1445/M × 100%, kwifomula ye-tne:
C - Ukuhlushwa kwesisombululo se-perchloric acid esijwayelekile kuma-moles ngelitha (mol/L)
V1 - Umthamo osetshenziselwa ukuhlukaniswa kwamasampula ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL).
I-Vo - Umthamo osetshenziselwa i-titration engenalutho ngesisombululo esijwayelekile se-perchloric acid, ngama-milliliters (mL);
M - Isisindo sesampula, ngamagremu (g).
0.1445: Isisindo esimaphakathi sama-amino acids esilingana no-1.00 mL wesisombululo se-perchloric acid esijwayelekile [c (HClO4) = 1.000 mol / L].
Isithasiselo C: Izindlela Zokunquma Izinga Lokuncishiswa Kwe-Sustar
Ukwamukelwa kwezindinganiso: Q/70920556 71-2024
1. Isimiso sokunquma (isibonelo se-Fe)
Ama-amino acid iron complexes anokuncibilika okuphansi kakhulu ku-anhydrous ethanol kanti ama-ion ensimbi akhululekile ayancibilika ku-anhydrous ethanol, umehluko ekuncibilikeni phakathi kwalawa amabili ku-anhydrous ethanol wasetshenziswa ukunquma izinga le-chelation lama-amino acid iron complexes.
2. Ama-reagent kanye nezixazululo
I-ethanol engenamanzi; okunye kufana nesigaba 4.5.2 ku-GB/T 27983-2011.
3. Izinyathelo zokuhlaziya
Yenza izivivinyo ezimbili ngesikhathi esisodwa. Kala u-0.1g wesampula omisiwe ku-103±2℃ ihora eli-1, ulingane no-0.0001g, engeza u-100mL we-ethanol engenamanzi ukuze uncibilikise, hlunga, hlunga izinsalela ezihlanziwe nge-100mL ye-ethanol engenamanzi okungenani izikhathi ezintathu, bese udlulisela izinsalela ebhodleleni eliyindilinga elingu-250mL, engeza u-10mL wesisombululo se-sulfuric acid ngokwesigaba 4.5.3 ku-GB/T27983-2011, bese wenza izinyathelo ezilandelayo ngokwesigaba 4.5.3 esithi “Shisa ukuze uncibilikise bese uvumela ukuthi kuphole” ku-GB/T27983-2011. Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
4. Ukunqunywa kokuqukethwe kwensimbi okuphelele
4.1 Isimiso sokunquma sifana nesigaba 4.4.1 ku-GB/T 21996-2008.
4.2. Ama-reagent kanye nezixazululo
4.2.1 I-asidi exubile: Engeza u-150mL we-sulfuric acid kanye no-150mL we-phosphoric acid ku-700mL wamanzi bese uxuba kahle.
4.2.2 Isixazululo senkomba ye-Sodium diphenylamine sulfonate: 5g/L, esilungiselelwe ngokwe-GB/T603.
4.2.3 Isixazululo se-titration esijwayelekile se-Cerium sulfate: ukuhlushwa c [Ce (SO4) 2] = 0.1 mol/L, okulungiselelwe ngokwe-GB/T601.
4.3 Izinyathelo zokuhlaziya
Yenza izivivinyo ezimbili ngesikhathi esisodwa. Kala u-0.1g wesampula, ofanele u-020001g, faka ebhodleleni eliyindilinga elingu-250mL, engeza u-10mL we-asidi exubile, ngemva kokuncibilika, engeza u-30ml wamanzi kanye namaconsi angu-4 esisombululo se-sodium dianiline sulfonate indicator, bese wenza lezi zinyathelo ezilandelayo ngokwesigaba 4.4.2 ku-GB/T21996-2008. Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
4.4 Ukumelwa kwemiphumela
Okuqukethwe kwensimbi okuphelele X1 kwe-amino acid iron complexes ngokwengxenye yensimbi, inani elivezwe ku-%, kubalwe ngokwefomula (1):
X1=(V-V0)×C×M×10-3×100
Kufomula: V - ivolumu yesisombululo esijwayelekile se-cerium sulfate esisetshenziswayo ukuze kuthathwe isixazululo sokuhlola, i-mL;
Isixazululo esijwayelekile se-cerium sulfate esisetshenziswa ekulinganisweni kwesisombululo esingenalutho, i-mL;
C - Ukuhlushwa kwangempela kwesisombululo esijwayelekile se-cerium sulfate, i-mol/L
5. Ukubalwa kokuqukethwe kwensimbi kuma-chelates
Okuqukethwe kwensimbi X2 ku-chelate ngokwengxenye yesisindo sensimbi, inani elivezwe ku-%, kubalwe ngokwefomula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Kufomula: V1 - ivolumu yesisombululo esijwayelekile se-cerium sulfate esisetshenziswayo ukuze kuthathwe isixazululo sokuhlola, i-mL;
Isixazululo esijwayelekile se-cerium sulfate esisetshenziswa ekulinganisweni kwesisombululo esingenalutho, i-mL;
C - Ukuhlushwa kwangempela kwesisombululo esijwayelekile se-cerium sulfate, i-mol/L;
0.05585 - isisindo sensimbi yensimbi evezwe ngamagremu alingana no-1.00 mL wesisombululo esijwayelekile se-cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1 - Isisindo sesampula, g. Thatha isilinganiso sezibalo semiphumela yokunquma ehambisanayo njengemiphumela yokunquma, futhi umehluko ophelele wemiphumela yokunquma ehambisanayo awudluli ku-0.3%.
6. Ukubalwa kwezinga le-chelation
Izinga le-Chelation X3, inani elivezwe ku-%, X3 = X2/X1 × 100
Isithasiselo C: Izindlela Zokunquma Izinga Lokuncishiswa Kwe-Zinpro
Ukwamukelwa kwendinganiso: Q/320205 KAVNO7-2016
1. Ama-reagent nezinto zokwakha
a) I-glacial acetic acid: ihlanzekile ngokwezibalo; b) I-Perchloric acid: 0.0500mol/L; c) Inkomba: Inkomba ye-crystal violet engu-0.1% (i-glacial acetic acid)
2. Ukunqunywa kwama-amino acid amahhala
2.1 Amasampula omisiwe ku-80°C ihora eli-1.
2.2 Beka isampula esitsheni esomile ukuze iphole ngokwemvelo kuze kufike ekushiseni kwegumbi noma iphole kuze kufike ekushiseni okusebenzisekayo.
2.3 Kala cishe u-0.1 g wesampula (onembile kuya ku-0.001 g) ebhodleleni eliyindilinga elomile elingu-250 mL
2.4 Qhubekela phambili ngokushesha esinyathelweni esilandelayo ukuze ugweme ukuthi isampula ingamunci umswakama ozungezile.
2.5 Engeza u-25mL we-glacial acetic acid bese uxuba kahle isikhathi esingaphezu kwemizuzu emi-5.
2.6 Engeza amaconsi amabili esibonakaliso se-crystal violet.
2.7 I-Titrate enesisombululo se-titration esijwayelekile esingu-0.0500mol/L (±0.001) se-perchloric acid kuze kube yilapho isisombululo sishintsha kusuka konsomi sibe luhlaza okwemizuzu engu-15 ngaphandle kokushintsha umbala njengendawo yokugcina.
2.8 Qopha umthamo wesisombululo esijwayelekile esisetshenzisiwe.
2.9 Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
3. Ukubala kanye nemiphumela
Okuqukethwe kwe-amino acid yamahhala u-X ku-reagent kuvezwa njengengxenye yesisindo (%), kubalwa ngokwefomula (1): X=C×(V1-V0) ×0.1445/M×100%........(1)
Kufomula: C - ukuhlushwa kwesisombululo se-perchloric acid esijwayelekile kuma-moles ngelitha (mol/L)
V1 - Umthamo osetshenziselwa ukuhlukaniswa kwamasampula ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL).
I-Vo - Umthamo osetshenziselwa i-titration engenalutho ngesisombululo esijwayelekile se-perchloric acid, ngama-milliliters (mL);
M - Isisindo sesampula, ngamagremu (g).
0.1445 - Isisindo esimaphakathi sama-amino acids esilingana no-1.00 mL wesisombululo se-perchloric acid esijwayelekile [c (HClO4) = 1.000 mol / L].
4. Ukubalwa kwezinga le-chelation
Izinga le-chelation lesampula livezwa njengengxenyana yesisindo (%), ebalwa ngokwefomula (2): izinga le-chelation = (okuqukethwe kwe-amino acid iyonke - okuqukethwe kwe-amino acid yamahhala)/okuqukethwe kwe-amino acid iyonke × 100%.
Isikhathi sokuthunyelwe: Septhemba 17-2025
