2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Izinto ezijwayelekile ezisetshenziswa ku-calibration curve yokusatshalaliswa kwesisindo sama-molecule okuhlobene: i-insulin, i-mycopeptides, i-glycine-glycine-tyrosine-arginine, i-glycine-glycine-glycine
3 Ithuluzi kanye nemishini
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Sekukonke, isilinganiso sama-amino acid emikhiqizweni kaSustar siphezulu kunesemikhiqizo kaZinpro.
Ingxenye 8 Imiphumela yokusetshenziswa
Imiphumela yemithombo ehlukene yamaminerali amancane ekusebenzeni kahle kokukhiqiza kanye nekhwalithi yamaqanda ezinkukhu ezibekela amaqanda esikhathini sokubeletha sekwephuzile
Inqubo Yokukhiqiza
Ubuchwepheshe be-chelation obuqondiwe
Ubuchwepheshe bokushefa i-emulsification
Ubuchwepheshe bokufutha nokomisa ngengcindezi
Ubuchwepheshe bokuqandisa nokususa umswakama
Ubuchwepheshe obuthuthukisiwe bokulawula imvelo
Isithasiselo A: Izindlela Zokunquma Ukusatshalaliswa Kwesisindo Sama-peptide Esihlobene Nama-molecule
Ukwamukelwa kwendinganiso: GB/T 22492-2008
1 Isimiso Sokuhlola:
Kwanqunywa yi-chromatography yokuhlunga ijeli esebenza kahle kakhulu. Okusho ukuthi, kusetshenziswa i-porous filler njengesigaba esimile, ngokusekelwe kumehluko ngobukhulu besisindo sama-molecule esihlobene sezingxenye zesampula zokuhlukanisa, ezitholwe ku-peptide bond ye-ultraviolet absorption wavelength engu-220nm, kusetshenziswa isofthiwe yokucubungula idatha ezinikele yokunquma ukusatshalaliswa kwesisindo sama-molecule esihlobene yi-gel filtration chromatography (okungukuthi, isofthiwe ye-GPC), ama-chromatogram kanye nedatha yawo kwacutshungulwa, kwabalwa ukuthola usayizi wesisindo sama-molecule esihlobene se-soybean peptide kanye nobubanzi bokusatshalaliswa.
2. Ama-reagent
Amanzi okuhlola kufanele ahlangabezane nencazelo yamanzi esibili ku-GB/T6682, ukusetshenziswa kwama-reagent, ngaphandle kwemibandela ekhethekile, kuhlanzekile ngokuhlaziya.
2.1 Ama-reagent afaka i-acetonitrile (emsulwa ngokwe-chromatographic), i-trifluoroacetic acid (emsulwa ngokwe-chromatographic),
2.2 Izinto ezijwayelekile ezisetshenziswa ku-calibration curve yokusatshalaliswa kwesisindo sama-molecule okuhlobene: i-insulin, i-mycopeptides, i-glycine-glycine-tyrosine-arginine, i-glycine-glycine-glycine
3 Ithuluzi kanye nemishini
3.1 I-Chromatograph Yoketshezi Esebenza Kakhulu (i-HPLC): indawo yokusebenza ye-chromatographic noma i-integrator ene-UV detector kanye nesofthiwe yokucubungula idatha ye-GPC.
3.2 Iyunithi yokuhlunga nokukhipha igesi yesigaba esiphathwayo.
3.3 Ibhalansi ye-elekthronikhi: inani eliphelele 0.000 1g.
Izinyathelo zokusebenza ezi-4
4.1 Izimo ze-Chromatographic kanye nokuhlolwa kokuzivumelanisa nesistimu (izimo zokubhekisela)
- 4.1.1 Ikholomu ye-Chromatographic: I-TSKgelG2000swxl300 mm×7.8 mm (ububanzi bangaphakathi) noma amanye amakholomu ejeli ohlobo olufanayo anokusebenza okufanayo okufanelekela ukunqunywa kwamaprotheni nama-peptide.
- 4.1.2 Isigaba sokuhamba: I-Acetonitrile + amanzi + i-trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Ubude be-wavelength bokuthola: 220 nm.
- 4.1.4 Izinga lokugeleza: 0.5 mL/min.
- 4.1.5 Isikhathi sokuthola: imizuzu engama-30.
- 4.1.6 Umthamo womjovo wesampula: 20μL.
- 4.1.7 Izinga lokushisa lekholomu: izinga lokushisa legumbi.
- 4.1.8 Ukuze kwenziwe uhlelo lwe-chromatographic luhlangabezane nezidingo zokuthola, kwabekwa ukuthi ngaphansi kwezimo ze-chromatographic ezingenhla, ukusebenza kahle kwekholomu ye-gel chromatographic, okungukuthi, inani lamapuleti (N), kwakungengaphansi kuka-10000 okubalwa ngokusekelwe eziqongweni ze-tripeptide standard (Glycine-Glycine-Glycine).
- 4.2 Ukukhiqizwa kwama-curve ajwayelekile esisindo sama-molecule ahlobene
- Izixazululo ezijwayelekile ze-peptide ezilinganiselwe ze-molecule mass peptide ezingenhla ezine-mass concentration engu-1 mg / mL zalungiswa ngokufanisa isigaba esiphathwayo, zaxutshwa ngesilinganiso esithile, zabe sezihlungwa nge-organic phase membrane enosayizi we-pore ongu-0.2 μm ~ 0.5 μm zafakwa kusampula, kwabe sekutholwa ama-chromatograms ezindinganiso. Ama-relative molecular mass calibration curves kanye nezibalo zawo zatholakala ngokuhlela i-logarithm ye-relative molecular mass ngokumelene nesikhathi sokugcina noma ngokuhlehla okuqondile.
4.3 Ukwelashwa kwesampula
Kala kahle isampula engu-10mg ebhodleleni elinomthamo ongu-10mL, engeza isigaba esincane esihambayo, ukushukunyiswa kwe-ultrasonic imizuzu eyi-10, ukuze isampula ichitheke ngokuphelele futhi ixutshwe, ixutshwe nesigaba esihambayo esikalini, bese ihlungwa nge-membrane yesigaba se-organic enosayizi wembobo ongu-0.2μm ~ 0.5μm, bese i-filtrate ihlaziywe ngokwezimo ze-chromatographic ku-A.4.1.
- 5. Ukubalwa kokusatshalaliswa kwesisindo sama-molecule okuhlobene
- Ngemva kokuhlaziya ikhambi lesampula elilungiselelwe ku-4.3 ngaphansi kwezimo ze-chromatographic zika-4.1, isisindo sama-molecule esihlobene sesampula kanye nobubanzi baso bokusatshalaliswa kungatholakala ngokufaka idatha ye-chromatographic yesampula ku-calibration curve 4.2 ngesofthiwe yokucubungula idatha ye-GPC. Ukusatshalaliswa kwesisindo sama-molecule esihlobene sama-peptide ahlukene kungabalwa ngendlela yokujwayeza indawo ephakeme, ngokwefomula: X=A/A inani ×100
- Kufomula: X - Ingxenye yesisindo se-peptide yesisindo sama-molecule esihlobene ku-peptide iyonke kusampula, %;
- A - Indawo ephakeme ye-peptide yesisindo sama-molecule esihlobene;
- Isamba A - isamba sezindawo eziphakeme ze-peptide ngayinye yesisindo sama-molecule esihlobene, esibalwa endaweni eyodwa yedesimali.
- 6 Ukuphindaphinda
- Umehluko ophelele phakathi kwezinqumo ezimbili ezizimele ezitholwe ngaphansi kwezimo zokuphindaphindeka akufanele udlule u-15% wesilinganiso sezibalo sezinqumo ezimbili.
- Isithasiselo B: Izindlela Zokunquma Ama-Amino Acids Amahhala
- Ukwamukelwa kwendinganiso: Q/320205 KAVN05-2016
- 1.2 Ama-reagent nezinto zokwakha
- I-acetic acid ye-glacial: ihlanzekile ngokwezibalo
- I-Perchloric acid: 0.0500 mol/L
- Inkomba: Inkomba ye-crystal violet engu-0.1% (i-glacial acetic acid)
- 2. Ukunqunywa kwama-amino acid amahhala
Amasampula omisiwe ku-80°C ihora eli-1.
Beka isampula esitsheni esomile ukuze iphole ngokwemvelo kuze kufike ekushiseni kwegumbi noma iphole kuze kufike ekushiseni okusebenzisekayo.Kala cishe u-0.1 g wesampula (onembile kuya ku-0.001 g) ebhodleleni eliyindilinga elomile elingu-250 mL.Qhubeka ngokushesha uye esinyathelweni esilandelayo ukuze ugweme ukuthi isampula ingamunci umswakama ozungezileEngeza u-25 mL we-glacial acetic acid bese uxuba kahle kungabi ngaphezu kwemizuzu emi-5.Engeza amaconsi amabili esibonakaliso se-crystal violetI-Titrate enesisombululo se-titration esijwayelekile esingu-0.0500 mol / L (±0.001) se-perchloric acid kuze kube yilapho isisombululo sishintsha kusuka kobubende kuya ekugcineni.
Qopha umthamo wesisombululo esijwayelekile esisetshenzisiwe.
- Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
- 3. Ukubala kanye nemiphumela
- Okuqukethwe kwe-amino acid yamahhala u-X ku-reagent kuvezwa njengengxenye enkulu (%) futhi kubalwa ngokwefomula: X = C × (V1-V0) × 0.1445/M × 100%, kwifomula ye-tne:
- C - Ukuhlushwa kwesisombululo se-perchloric acid esijwayelekile kuma-moles ngelitha (mol/L)
- V1 - Umthamo osetshenziselwa ukuhlukaniswa kwamasampula ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL).
- I-Vo - Umthamo osetshenziselwa i-titration engenalutho ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL);
M - Isisindo sesampula, ngamagremu (g).
| 0.1445: Isisindo esimaphakathi sama-amino acids esilingana no-1.00 mL wesisombululo se-perchloric acid esijwayelekile [c (HClO4) = 1.000 mol / L]. | 4.2.3 Isixazululo se-titration esijwayelekile se-Cerium sulfate: ukuhlushwa c [Ce (SO4) 2] = 0.1 mol/L, okulungiselelwe ngokwe-GB/T601. | |
| Ukwamukelwa kwezindinganiso: Q/70920556 71-2024 | 1. Isimiso sokunquma (isibonelo se-Fe) | Ama-amino acid iron complexes anokuncibilika okuphansi kakhulu ku-anhydrous ethanol kanti ama-ion ensimbi akhululekile ayancibilika ku-anhydrous ethanol, umehluko ekuncibilikeni phakathi kwalawa amabili ku-anhydrous ethanol wasetshenziswa ukunquma izinga le-chelation lama-amino acid iron complexes. |
| Kufomula: V1 - ivolumu yesisombululo esijwayelekile se-cerium sulfate esisetshenziswayo ukuze kuthathwe isixazululo sokuhlola, i-mL; | I-ethanol engenamanzi; okunye kufana nesigaba 4.5.2 ku-GB/T 27983-2011. | 3. Izinyathelo zokuhlaziya |
| Yenza izivivinyo ezimbili ngesikhathi esisodwa. Kala u-0.1g wesampula omisiwe ku-103±2℃ ihora eli-1, ulingane no-0.0001g, engeza u-100mL we-ethanol engenamanzi ukuze uncibilikise, hlunga, hlunga izinsalela ezihlanziwe nge-100mL ye-ethanol engenamanzi okungenani izikhathi ezintathu, bese udlulisela izinsalela ebhodleleni eliyindilinga elingu-250mL, engeza u-10mL wesisombululo se-sulfuric acid ngokwesigaba 4.5.3 ku-GB/T27983-2011, bese wenza izinyathelo ezilandelayo ngokwesigaba 4.5.3 esithi “Shisa ukuze uncibilikise bese uvumela ukuthi kuphole” ku-GB/T27983-2011. Yenza isivivinyo esingenalutho ngesikhathi esifanayo. | 4. Ukunqunywa kokuqukethwe kwensimbi okuphelele | 4.1 Isimiso sokunquma sifana nesigaba 4.4.1 ku-GB/T 21996-2008. |
4.2. Ama-reagent kanye nezixazululo
| 4.2.1 I-asidi exubile: Engeza u-150mL we-sulfuric acid kanye no-150mL we-phosphoric acid ku-700mL wamanzi bese uxuba kahle. | 4.2.2 Isixazululo senkomba ye-Sodium diphenylamine sulfonate: 5g/L, esilungiselelwe ngokwe-GB/T603. | 4.2.3 Isixazululo se-titration esijwayelekile se-Cerium sulfate: ukuhlushwa c [Ce (SO4) 2] = 0.1 mol/L, okulungiselelwe ngokwe-GB/T601. | |
| 4.3 Izinyathelo zokuhlaziya | Yenza izivivinyo ezimbili ngesikhathi esisodwa. Kala u-0.1g wesampula, ofanele u-020001g, faka ebhodleleni eliyindilinga elingu-250mL, engeza u-10mL we-asidi exubile, ngemva kokuncibilika, engeza u-30ml wamanzi kanye namaconsi angu-4 esisombululo se-sodium dianiline sulfonate indicator, bese wenza lezi zinyathelo ezilandelayo ngokwesigaba 4.4.2 ku-GB/T21996-2008. Yenza isivivinyo esingenalutho ngesikhathi esifanayo. | 4.4 Ukumelwa kwemiphumela | Okuqukethwe kwensimbi okuphelele X1 kwe-amino acid iron complexes ngokwengxenye yensimbi, inani elivezwe ku-%, kubalwe ngokwefomula (1): |
| X1=(V-V0)×C×M×10-3×100 | Isixazululo esijwayelekile se-cerium sulfate esisetshenziswa ekulinganisweni kwesisombululo esingenalutho, i-mL; | Isixazululo esijwayelekile se-cerium sulfate esisetshenziswa ekulinganisweni kwesisombululo esingenalutho, i-mL; | C - Ukuhlushwa kwangempela kwesisombululo esijwayelekile se-cerium sulfate, i-mol/L5. Ukubalwa kokuqukethwe kwensimbi kuma-chelatesOkuqukethwe kwensimbi X2 ku-chelate ngokwengxenye yesisindo sensimbi, inani elivezwe ku-%, kubalwe ngokwefomula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Kufomula: V1 - ivolumu yesisombululo esijwayelekile se-cerium sulfate esisetshenziswayo ukuze kuthathwe isixazululo sokuhlola, i-mL; | Isixazululo esijwayelekile se-cerium sulfate esisetshenziswa ekulinganisweni kwesisombululo esingenalutho, i-mL;nom1 - Isisindo sesampula, g. Thatha isilinganiso sezibalo semiphumela yokunquma ehambisanayo njengemiphumela yokunquma, futhi umehluko ophelele wemiphumela yokunquma ehambisanayo awudluli ku-0.3%. | 0.05585 - isisindo sensimbi yensimbi evezwe ngamagremu alingana no-1.00 mL wesisombululo esijwayelekile se-cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Isisindo sesampula, g. Thatha isilinganiso sezibalo semiphumela yokunquma ehambisanayo njengemiphumela yokunquma, futhi umehluko ophelele wemiphumela yokunquma ehambisanayo awudluli ku-0.3%. | 6. Ukubalwa kwezinga le-chelationIzinga le-Chelation X3, inani elivezwe ku-%, X3 = X2/X1 × 100Isithasiselo C: Izindlela Zokunquma Izinga Lokuncishiswa Kwe-Zinpro |
Ukwamukelwa kwendinganiso: Q/320205 KAVNO7-2016
1. Ama-reagent nezinto zokwakha
a) I-glacial acetic acid: ihlanzekile ngokwezibalo; b) I-Perchloric acid: 0.0500mol/L; c) Inkomba: Inkomba ye-crystal violet engu-0.1% (i-glacial acetic acid)
2. Ukunqunywa kwama-amino acid amahhala
2.1 Amasampula omisiwe ku-80°C ihora eli-1.
2.2 Beka isampula esitsheni esomile ukuze iphole ngokwemvelo kuze kufike ekushiseni kwegumbi noma iphole kuze kufike ekushiseni okusebenzisekayo.
2.3 Kala cishe u-0.1 g wesampula (onembile kuya ku-0.001 g) ebhodleleni eliyindilinga elomile elingu-250 mL
2.4 Qhubekela phambili ngokushesha esinyathelweni esilandelayo ukuze ugweme ukuthi isampula ingamunci umswakama ozungezile.
2.5 Engeza u-25mL we-glacial acetic acid bese uxuba kahle isikhathi esingaphezu kwemizuzu emi-5.
2.6 Engeza amaconsi amabili esibonakaliso se-crystal violet.
2.7 I-Titrate enesisombululo se-titration esijwayelekile esingu-0.0500mol/L (±0.001) se-perchloric acid kuze kube yilapho isisombululo sishintsha kusuka konsomi sibe luhlaza okwemizuzu engu-15 ngaphandle kokushintsha umbala njengendawo yokugcina.
2.8 Qopha umthamo wesisombululo esijwayelekile esisetshenzisiwe.
2.9 Yenza isivivinyo esingenalutho ngesikhathi esifanayo.
- 3. Ukubala kanye nemiphumela
- IsiCatalan
- Physicochemical parameters
V1 - Umthamo osetshenziselwa ukuhlukaniswa kwamasampula ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL).
I-Vo - Umthamo osetshenziselwa i-titration engenalutho ngesisombululo se-perchloric acid esijwayelekile, ngama-milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Ikheli: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sifundazwe saseSichuan, eShayina
Ucingo: 86-18880477902
Imikhiqizo
Amaminerali angabonakali
- Amaminerali okulandelela e-organic
- IsiSwahili
- Isevisi eyenziwe ngokwezifiso
- Izixhumanisi ezisheshayo
Iphrofayela Yenkampani
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| IsiGujarati | Chofoza ukuze ubuze | © Ilungelo Lokushicilela - 2010-2025: Wonke Amalungelo Agodliwe. | Imephu yesayithi UKUSESHA OKUPHEZULU Ucingo |
| Ucingo | 86-18880477902 | IsiJavanese | I-imeyili I-Whatsapp |
| 8618880477902 | IsiShayina | IsiFulentshi | |
| Bird | IsiShayina | IsiFulentshi | IsiJalimane IsiSpanishi |
| Aquatic animals | IsiJapane | IsiKorea | Isi-Arabhu isiGreki |
| IsiTurkey | IsiNtaliyane | ||
| Ruminant animal g/head day | January 0.75 | Isi-Indonesia IsiBhunu IsiSwidi |
IsiPolish
- IsiBasque
- IsiCatalan
- Physicochemical parameters
IsiHindi
IsiLao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
IsiShona
IsiBulgaria
- IsiCebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- IsiCroatia
IsiDashi
| Application object | Isi-Urdu IsiVietnamese | Content in full-value feed (mg/kg) | Efficacy |
| IsiGujarati | IsiHaiti | IsiHausa | IsiKinyarwanda IsiHmong IsiHungary |
| Piglets and fattening pigs | Isi-Igbo | IsiJavanese | IsiKannada IsiKhmer IsiKurdi |
| IsiKyrgyz | IsiLatini | ||
| Bird | 300～400 | 45～60 | IsiMacedonia IsiMalay IsiMalayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
IsiNorway
- IsiPashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
IsiSerbia
IsiSuthu
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
IsiShona
IsiSindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
IsiSwahili
IsiTajik
IsiTamil
IsiTelugu
IsiThai
| Application object | Isi-Urdu IsiVietnamese | Content in full-value feed (mg/kg) | Efficacy |
| IsiYiddish | IsiYoruba | IsiZulu | IsiKinyarwanda Isi-Oriya Abantu baseTurkmen |
| Isi-Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
